#following script maps reads to brunello CRISPR gRNA design, and further normalizes reads counts for each gRNA
perl  ./BASICv4.pl   -g ./bowtie2_lib/new_brunello_gRNA.txt   -b ./bowtie2_lib/new_brunello_gRNA  -o 	output_name	 -n  40000000	 -f	../fastq_original/ARP1C/xxxxxx.fastq	
